{"product_id":"abcam-ab257185","title":"Abcam, ab257185, Human STAT2 knockout A549 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSTAT2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 19 bp deletion in exon2 and 1 bp deletion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 19 bp deletion in exon2 and 1 bp deletion in exon2.,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-STAT2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Signal Transducer and Activator of Transcription 2 commonly known as STAT2 is a protein with a molecular mass of approximately 97 kDa. It functions mainly as a transcription factor and is an essential mediator of the interferon (IFN) signaling pathway. STAT2 is expressed in various tissues including immune cells where it responds to external stimuli. It works by translocating to the nucleus upon phosphorylation subsequently influencing the expression of genes involved in immune response.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMembers of the STAT protein family including STAT2 play a central role in mediating immune responses. STAT2 often forms a complex with STAT1 and other proteins such as IRF9 to initiate the transcription of interferon-stimulated genes (ISGs). This complex known as the ISGF3 complex facilitates the cellular response to viral infections by promoting the expression of antiviral proteins that help modify the host cellular environment to resist viral replication.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe involvement of STAT2 is closely associated with the JAK-STAT signaling pathway and the interferon signaling pathway. In these pathways it interacts with proteins like JAK1 TYK2 and STAT1 playing a part in transmitting signals from the cell surface to the nucleus. Such pathways are fundamental to regulating the body's immune response to pathogens and managing cell growth and apoptosis in various cells.\u003cbr\u003e\nSTAT2 alterations have been linked to autoimmune diseases and viral infections. Disruptions in STAT2 function can impair the body's ability to combat viruses effectively making individuals more susceptible to infections. STAT2 mutations or dysregulations might also connect to disorders like Systemic Lupus Erythematosus where immune system malfunctions occur. The interactions of STAT2 with other proteins like STAT1 and IRF9 in disease contexts underline its importance in maintaining immune system balance.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845097083049,"sku":"ab257185","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab257185","provider":"Iright","version":"1.0","type":"link"}