{"product_id":"abcam-ab257665","title":"Abcam, ab257665, Human SESN2 (Sestrin-2) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSESN2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon4.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon4.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SESN2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSESN2 also known as Sestrin-2 or Hi95 is a stress-inducible protein that plays a role in cellular responses to oxidative stress. It has a molecular mass of approximately 60 kDa and is expressed in various tissues including the liver heart and muscle. Sestrin-2 acts as an antioxidant protein that helps reduce reactive oxygen species (ROS) and maintains cellular redox balance. It contains domains that directly interact with target proteins to regulate cellular activity enabling cells to respond efficiently to stress signals.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSestrin-2 regulates cell survival and homeostasis during oxidative stress conditions. It modulates the activity of key signaling pathways that control autophagy and cell growth. Sestrin-2 does not function alone but interacts with other proteins to form functional complexes. This allows it to fine-tune cellular processes and contributes to its role in promoting cell repair mechanisms. Its induction during stress conditions demonstrates its engagement in promoting cellular adaptation and resistance to damage.\u003cbr\u003e\nPathways\u003cbr\u003e\nSestrin-2 engages in signaling networks such as the AMPK and mTOR pathways. Its interaction with AMP-activated protein kinase (AMPK) triggers energy homeostasis and metabolic stress responses. It also negatively regulates the mammalian target of rapamycin (mTOR) to support autophagy and inhibit cell growth when necessary. Sestrin-2 therefore plays an important role in coordinating responses between these pathways ensuring optimal cellular function during periods of environmental stress or nutrient deprivation.\u003cbr\u003e\nSESN2 is implicated in conditions related to oxidative stress and metabolic dysregulation such as cancer and type 2 diabetes. Its involvement in cancer is connected to its regulatory role on the mTOR pathway often overactive in tumor growth. The protein’s modulation of AMPK-mTOR signaling can impact cancer cell survival and response to treatment. Additionally in diabetes Sestrin-2 plays a part in maintaining insulin sensitivity and glucose metabolism often disrupted in diabetic patients. Its ability to regulate oxidative stress and cellular metabolism draws a link between Sestrin-2 and these complex disorders emphasizing its potential as a therapeutic target.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845100163241,"sku":"ab257665","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab257665","provider":"Iright","version":"1.0","type":"link"}