{"product_id":"abcam-ab257798","title":"Abcam, ab257798, Human WHSC1 (NSD2) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nNSD2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWestern blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-NSD2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nWHSC1 also known as NSD2 is a histone methyltransferase enzyme involved in the methylation of lysine 36 on histone H3. This enzyme facilitates the addition of a methyl group which affects chromatin structure and gene expression. NSD2 is also called WHSC1L1 or Wolf-Hirschhorn syndrome candidate 1-like 1. It has a molecular weight around 153 kDa. Researchers observe the expression of WHSC1\/NSD2 predominantly in the nucleus but it is present in various tissues including lymphoid organs and certain types of cancer cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nWHSC1\/NSD2 functions as an epigenetic regulator modifying histones to modulate transcriptional activity. It exists as part of a larger protein complex that includes several chromatin-associated proteins. Its role in histone methylation influences gene expression impacting cell differentiation survival and proliferation. Through controlling gene transcription WHSC1\/NSD2 plays a significant role in development and cellular responses to environmental signals.\u003cbr\u003e\nPathways\u003cbr\u003e\nWHSC1\/NSD2 participates in key biological processes such as the cell cycle and DNA damage response pathways. It influences histone modification pathways by interacting with other proteins like SETD2 and H3K36me3 which are important for maintaining genomic integrity. In the DNA damage response NSD2 works together with proteins such as ATM coordinating repair mechanisms and cell cycle progression.\u003cbr\u003e\nAlterations in WHSC1\/NSD2 expression or function associate with multiple myeloma and Wolf-Hirschhorn syndrome. In multiple myeloma overexpression or mutations of WHSC1\/NSD2 lead to altered gene expression profiles and promote cancer cell proliferation. Within this context NSD2 often interacts with MMSET and FGFR3 proteins contributing to oncogenic transformation. Additionally mutations in WHSC1\/NSD2 link to Wolf-Hirschhorn syndrome a genetic condition characterized by growth delay and intellectual disability.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845112320169,"sku":"ab257798","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab257798","provider":"Iright","version":"1.0","type":"link"}