{"product_id":"abcam-ab257816","title":"Abcam, ab257816, Human ADRM1 (ARM-1) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nADRM1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 16 bp deletion in exon 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 16 bp deletion in exon 2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ADRM1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nADRM1 known also as ARM-1 or arm protein is an essential component of the proteasome system playing an important role in ubiquitin-proteasome pathway. Its molecular mass is about 42-46 kDa. ADRM1 protein is expressed across various tissues with significant presence in tissues requiring active protein degradation such as liver muscle and neurons. This protein interacts with other subunits in large protein complexes contributing to the regulation of protein turnover.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe ADRM1\/ARM-1 protein binds ubiquitinated substrates and shuttles them to the 26S proteasome for degradation. It acts as a receptor on the regulatory particle of the proteasome complex where it recruits deubiquitinating enzymes. This positions ADRM1 as an integral part of the protein degradation machinery ensuring removal of damaged misfolded or unnecessary proteins and maintaining cellular protein homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nADRM1 plays a significant role within the ubiquitin-proteasome pathway which is important for protein catabolism. It partners with regulatory proteins like Rpn13 to mediate the recognition and processing of polyubiquitinated substrates. The efficient functioning of this pathway highlights its importance in regulating the cell cycle and various signaling pathways including NF-kB signaling which affects immune response and apoptosis.\u003cbr\u003e\nDysfunction of ADRM1 can lead to pathological consequences. Its aberrant activity links to cancer particularly in solid tumors due to its role in controlling the degradation of cell cycle proteins. Additionally alterations in ADRM1 functionality can affect Alzheimer's disease as proper protein turnover is important in preventing accumulation of protein aggregates. Interactions with proteins like USP14 and UCHL5 further illustrate ADRM1's involvement in these pathologies marking it as a potential therapeutic target.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845095411881,"sku":"ab257816","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab257816","provider":"Iright","version":"1.0","type":"link"}