{"product_id":"abcam-ab257840","title":"Abcam, ab257840, Human ARF5 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nARF5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 13 bp deletion in exon1 and 1 bp insertion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 13 bp deletion in exon1 and 1 bp insertion in exon1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ARF5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe ADP-ribosylation factor 5 (ARF5) protein also known by its primary name is an important part of the ARF family. It has a mass of around 20 kDa. ARF5 plays a role in regulating vesicle trafficking systems particularly linked with the Golgi apparatus. It is found broadly expressed in numerous tissues with high levels observed in the brain and testes. This small GTP-binding protein is associated with intracellular signaling cascades and cellular communication.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nARF5 is involved in modulating key cellular processes including membrane trafficking and lipid metabolism. It directly participates in the formation of vesicle coats by aiding the recruitment of coat protein complexes. ARF5 also interacts within cellular complexes impacting the transport of cargo in the secretory pathway. It also affects actin cytoskeleton remodeling thereby influencing cell shape and motility.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe ARF5 protein contributes to both the COPI and clathrin-coated vesicle formation pathways. It functions within intricate signaling networks coordinating with other small GTPases such as ARF1. ARF5's activity links to pivotal roles within these pathways affecting protein and lipid sorting. These pathways are vital for maintaining cellular homeostasis and facilitating effective intracellular transport.\u003cbr\u003e\nARF5's dysfunction has been linked to neurodegenerative disorders including Alzheimer's disease. Variations in the expression or mutations of ARF5 might contribute to the disease pathology by impairing vesicular transport and potentially interacting with proteins like tau. Furthermore ARF5 may relate to metabolic syndromes due to its involvement in lipid metabolism with implications for body fat distribution and lipid-related diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845174349993,"sku":"ab257840","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab257840","provider":"Iright","version":"1.0","type":"link"}