{"product_id":"abcam-ab257888","title":"Abcam, ab257888, Human CECR5 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nHDHD5 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 17 bp deletion in exon 6 and 1 bp deletion in exon 6.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 17 bp deletion in exon 6 and 1 bp deletion in exon 6.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-HDHD5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCECR5 also known as Cat Eye Syndrome Critical Region Candidate 5 encodes a protein with a mass of about 48 kDa. This protein shows expression in multiple tissues such as the brain kidney and liver. Its mechanical role involves binding interactions that help in signaling and cellular communication processes. The protein's structure enables it to participate effectively in these interactions making it a point of interest for further study in these specific tissues.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCECR5 serves an important function in cellular signaling networks and is not typically found as part of a larger complex. The protein plays a role in modulating certain cellular responses possibly impacting processes like differentiation and growth. Researchers have identified CECR5 involvement in cellular mechanisms that govern normal development and maintenance hinting at its broader influence in cellular homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nCECR5 engages in the MAPK signaling pathway which is instrumental in transferring extracellular signals to intracellular responses. The MAPK pathway involves proteins like ERK and JNK with which CECR5 may interact or have regulatory effects. Additionally it influences signaling via the PI3K-AKT pathway a pathway that controls many aspects of cell survival growth and metabolism. This association suggests CECR5's potential roles in important cellular activities and regulatory mechanisms.\u003cbr\u003e\nAlterations in CECR5 expression or function relate to Cat Eye Syndrome and possibly neurodevelopmental disorders. In Cat Eye Syndrome the overexpression or structural changes of CECR5 may disrupt normal development alongside chromosomal duplications involving the region. Connections also appear between CECR5 and other proteins like PAX6 in the context of its role in developmental processes. Understanding these relationships further explains CECR5's impact on these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845096001705,"sku":"ab257888","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab257888","provider":"Iright","version":"1.0","type":"link"}