{"product_id":"abcam-ab257968","title":"Abcam, ab257968, Human GLYR1 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nGLYR1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 10 bp insertion in exon 3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 10 bp insertion in exon 3.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-GLYR1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGLYR1 also known as glyoxylate reductase 1 homolog or NP60 is a 62 kDa protein. It is an NAD(P)H-dependent oxidoreductase and binds specifically to histones modifying their methylation status. GLYR1 shows expression in various tissues with higher levels noted in the kidney and liver. It plays a role in chromatin remodeling indicating its involvement in gene expression regulation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGLYR1 influences chromatin structure by regulating histone H3 lysine 36 trimethylation (H3K36me3). It interacts with lysine-specific demethylase 1 (LSD1) and partners within chromatin-modifying complexes. Through these interactions GLYR1 can impact transcriptional repression and activation. Its activity shows specific importance in controlling gene expression related to cell growth and differentiation.\u003cbr\u003e\nPathways\u003cbr\u003e\nGLYR1 interacts with components of the MAPK signaling pathway and the histone modification pathway. It works alongside proteins like LSD1 and histone deacetylases (HDACs) to modulate transcriptional outcomes. These interactions provide evidence of its role in transmitting extracellular signals to the nucleus ultimately influencing gene expression patterns.\u003cbr\u003e\nResearch links GLYR1 to cancer progression and inflammatory responses. Its overexpression or dysregulation has associations with tumors where alteration of histone marks leads to aberrant gene expression. GLYR1 also connects with proteins like HDACs in these diseases contributing to the modulation of cell cycle and apoptosis which are critical for cancer development.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845100097705,"sku":"ab257968","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab257968","provider":"Iright","version":"1.0","type":"link"}