{"product_id":"abcam-ab257975","title":"Abcam, ab257975, Human GNS knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nGNS KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-GNS, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe GNS protein also known as glucosamine (N-acetyl)-6-sulfatase is an enzyme with significant roles in the breakdown of glycosaminoglycans. Its molecular weight is around 63 kDa. GNS is frequently expressed in lysosomal compartments where it catalyzes the cleavage of sulfate groups from N-acetylglucosamine residues in heparan sulfate and other similar molecules. This process is critical for the degradation and recycling of complex carbohydrates in cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGlucosamine (N-acetyl)-6-sulfatase activity influences cellular homeostasis by acting within the lysosomal hydrolase family. GNS participates in the breakdown and metabolism of glycosaminoglycans important for maintaining cellular integrity and signaling functions. It operates as a standalone enzyme rather than part of a larger complex. Through its enzymatic function GNS helps prevent the accumulation of partially degraded molecules inside lysosomes.\u003cbr\u003e\nPathways\u003cbr\u003e\nGlucosamine (N-acetyl)-6-sulfatase activity plays a role in lysosomal storage pathways and in the catabolic processes of heparan sulfate. This enzyme works alongside other lysosomal proteins like iduronate sulfatase and alpha-L-iduronidase which are also involved in glycosaminoglycan degradation pathways. Proper function of these pathways is important for cellular waste management and nutrient recycling.\u003cbr\u003e\nAltered GNS function relates to conditions such as mucopolysaccharidosis type IIID (Sanfilippo syndrome type D) and some forms of lysosomal storage diseases. Deficiencies in GNS disrupt normal lysosomal breakdown of glycosaminoglycans leading to accumulations that can cause cellular damage. This disorder often involves other metabolic proteins like heparan N-sulfatase and alpha-N-acetylglucosaminidase. Understanding the role of GNS offers insights into potential therapeutic approaches for related metabolic disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845087514793,"sku":"ab257975","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab257975","provider":"Iright","version":"1.0","type":"link"}