{"product_id":"abcam-ab258123","title":"Abcam, ab258123, Human PLSCR1 (Scramblase 1) knockout A549 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nPLSCR1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon4 and 1 bp insertion in exon4.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon4 and 1 bp insertion in exon4.,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PLSCR1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nScramblase 1 also known as PLSCR1 is a protein involved in the translocation of phospholipids between the inner and outer leaflets of the lipid bilayer. This protein has a molecular mass of approximately 37 kDa. PLSCR1 is found in various tissues and cells with notable expression in the hematopoietic system. As a type II membrane protein Scramblase 1 plays a role in maintaining lipid asymmetry especially during cellular activation or apoptosis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nScramblase 1 mediates phospholipid scrambling in response to increased intracellular calcium levels facilitating changes in cell membrane morphology. It performs this function independently and as part of specific protein complexes that regulate membrane curvature and vesicle fusion. The role of PLSCR1 extends beyond membrane activities; it has been linked to signal transduction and the immune response suggesting a broader impact on cellular dynamics.\u003cbr\u003e\nPathways\u003cbr\u003e\nScramblase 1 influences critical biological processes like blood coagulation and apoptosis. It interacts with proteins such as annexin V which stabilizes membrane phospholipids and regulates cell death pathways. In the coagulation cascade PLSCR1 has been shown to interact with factors involved in platelet activation demonstrating its regulatory role in maintaining hemostasis and tissue homeostasis.\u003cbr\u003e\nScramblase 1 has notable implications in cancer and autoimmune diseases. Altered expression or mutation of PLSCR1 links directly to the progression of certain cancers often associating with proteins like p53 which plays an important role in cell cycle regulation and tumor suppression. In autoimmune disorders Scramblase 1’s activity affects the balance of immune cell signaling potentially linking it with proteins involved in inflammatory responses such as cytokines. Understanding these connections provides insights into potential therapeutic targets and diagnostic markers for these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845130768553,"sku":"ab258123","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258123","provider":"Iright","version":"1.0","type":"link"}