{"product_id":"abcam-ab258160","title":"Abcam, ab258160, Human RAB14 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nRAB14 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 2 bp insertion in exon 4 and Insertion of the selection cassette in exon 4.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 2 bp insertion in exon 4 and Insertion of the selection cassette in exon 4.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RAB14, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRAB14 also known as Ras-related protein Rab-14 is part of the Rab family of small GTPases. It has a molecular mass of approximately 23 kDa and functions to regulate membrane trafficking between the Golgi apparatus and endosomes. RAB14 mediates vesicle movement and membrane fusion playing an important role in endocytic and secretory pathways. It is expressed in various tissues and cells including the brain heart and lungs suggesting its involvement in diverse physiological processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRAB14 orchestrates intracellular transport processes important for cell homeostasis. It does not act alone but often forms complexes with other proteins like protein phosphatase 1 and interactions involving KIF3A and DVL. These interactions enable RAB14 to influence cargo sorting and organelle morphology which is essential for correct protein trafficking and recycling in the cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nScientists have placed RAB14 in relation to the regulation of membrane trafficking and cell signaling. RAB14 influences the pathways of endocytosis and trans-Golgi network trafficking. It has associations with proteins such as RAB11 and RAB5 in these pathways. These proteins work in coordination to ensure effective sorting and distribution of intracellular materials affecting various cellular processes.\u003cbr\u003e\nMutations or dysregulation of RAB14 associate with cancer progression and metabolic disorders. Abnormal RAB14 activity influences tumorigenesis by affecting cell migration and invasion in part through interactions with proteins such as Rac1. Additionally RAB14 plays a role in insulin signaling pathways linking it to metabolic disorders related to insulin resistance and glucose homeostasis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845131522217,"sku":"ab258160","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258160","provider":"Iright","version":"1.0","type":"link"}