{"product_id":"abcam-ab258194","title":"Abcam, ab258194, Human SLC2A8 (Glucose Transporter 8) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSLC2A8 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SLC2A8, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGlucose Transporter 8 also known as GLUT8 is a member of the sugar transporter family. This protein plays a mechanical role in transferring glucose across cellular membranes. GLUT8 operates with facilitative transport ensuring glucose entry into cells. It has a molecular mass of around 55 kDa. Expression sites for GLUT8 include tissues like the testis brain and placenta. This specific transporter assists in maintaining glucose homeostasis critical for cell metabolism and energy production.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGLUT8 facilitates the uptake of glucose into cells supporting cellular survival and energetic demands. It does not appear to be part of any large protein complex but operates independently to bring glucose into the intracellular environment. This functionality underpins its involvement in energy regulation influencing cellular respiration and growth by ensuring glucose availability especially in specialized tissues such as the brain and placenta.\u003cbr\u003e\nPathways\u003cbr\u003e\nGLUT8 acts within the insulin signaling and glucose transport pathways. The transporter integrates with the insulin-responsive network where it responds to insulin stimulation to increase glucose uptake in cells expressing it similar to insulin-regulated transporters like GLUT4. GLUT8's functionality ensures effective glucose utilization directly linking its transport activity with energy metabolism pathways.\u003cbr\u003e\nGLUT8's role connects to metabolic dysfunction and neurological conditions. Research shows a connection to diabetes primarily through its responsive nature to insulin which affects glucose homeostasis. Additionally GLUT8 is linked to Alzheimer's disease with potential interactions with amyloid precursor proteins and insulin signaling disruptions. These connections highlight GLUT8's relevance to neurological and energy balance disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855781384361,"sku":"ab258194","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258194","provider":"Iright","version":"1.0","type":"link"}