{"product_id":"abcam-ab258207","title":"Abcam, ab258207, Human SRGN (Serglycin) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSRGN KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1 and 5 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1 and 5 bp deletion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SRGN, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSerglycin also known as hematopoietic proteoglycan core protein is a critical component of the extracellular matrix with a mass of approximately 17 kDa. It plays an important mechanical role by serving as a scaffold for the attachment of glycosaminoglycan (GAG) chains. These chains help serglycin mediate the storage and secretion of various bioactive molecules. It is expressed predominantly in hematopoietic cells including mast cells neutrophils and macrophages and also in endothelial cells serving diverse biological functions through its ability to interact with a wide variety of molecules.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSerglycin acts as a mediator of cell granule storage and release processes. It is essential in the storage of secretory granules in mast cells and is involved in the process of degranulation where it participates in inflammation response and hemostasis. Serglycin associates with various proteins forming complexes with them to facilitate the storage of inflammatory mediators such as histamine and proteases. Its ability to bind to and sequester these bioactive molecules plays significant roles in the regulation of both innate and adaptive immune responses.\u003cbr\u003e\nPathways\u003cbr\u003e\nSerglycin is an integral part of the inflammatory response pathway and the coagulation cascade. In the inflammatory pathway it modulates the release and activity of key cytokines and chemokines thereby participating in the regulation of immune cell recruitment and activation. In the coagulation cascade it interacts with protease inhibitors and various clotting factors to regulate coagulation processes. Serglycin's relationships with proteins like cathepsin G and elastase further illustrate its involvement in these pathways enhancing the modulation of inflammatory and hemostatic responses.\u003cbr\u003e\nAberrations in Serglycin function or expression have been linked to several conditions including asthma and certain types of leukemia. In asthma it may influence the severity of the disease through its role in the storage and release of inflammatory mediators in mast cells. In leukemia especially acute myeloid leukemia alterations in Serglycin expression can affect leukemic cell survival and proliferation. These links between Serglycin and disease states often involve interaction with other proteins like chymase and platelet factor 4 which contribute to disease processes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845188112553,"sku":"ab258207","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258207","provider":"Iright","version":"1.0","type":"link"}