{"product_id":"abcam-ab258409","title":"Abcam, ab258409, Human EMP1 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nEMP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2 and 2 bp insertion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2 and 2 bp insertion in exon2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-EMP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEMP-1 also known as epithelial membrane protein-1 is a transmembrane protein with a molecular weight of approximately 22 kDa. It is expressed in a variety of tissues including epithelial cells and is highly present in the skin lungs and certain immune cells. EMP-1 belongs to the peripheral myelin protein 22 (PMP22) family sharing structural homology with other tetraspan membrane proteins. The protein serves as a cellular marker for epithelial and some mesenchymal-derived tissues.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEMP-1 plays a significant role in cell-cell interactions and maintenance of cellular structures. It acts as part of a larger protein complex that contributes to cellular differentiation and tissue integrity. EMP-1 influences cell proliferation and modulates cell morphology which is essential during development and tissue repair. This protein also has a function in signal transduction where it may impact cellular responses to external stress.\u003cbr\u003e\nPathways\u003cbr\u003e\nEMP-1 integrates into multiple signaling pathways such as the MAPK\/ERK pathway and the Wnt signaling pathway. These pathways are important for processes like cell growth apoptosis and differentiation. EMP-1 interacts with proteins such as β-catenin and ERK thereby influencing the transcriptional regulation of genes involved in cell survival and growth. The protein’s presence in these pathways highlights its role in cellular physiological adaptations.\u003cbr\u003e\nEMP-1 has been associated with cancers such as lung cancer and head and neck squamous cell carcinoma. Its expression correlates with tumor progression and metastasis. EMP-1 interacts with proteins like E-cadherin potentially disrupting cell adhesion therefore facilitating cancer cell invasion. Understanding the role of EMP-1 in these diseases could help in developing therapeutic strategies targeting its molecular interactions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845132832937,"sku":"ab258409","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258409","provider":"Iright","version":"1.0","type":"link"}