{"product_id":"abcam-ab258417","title":"Abcam, ab258417, Human ETV6 (Tel) knockout A549 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nETV6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon5.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon5.,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ETV6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nETV6 also known as Tel is a transcription factor encoded by the ETV6 gene. It weighs approximately 57 kDa. The protein is expressed in various tissues including hematopoietic cells which are cells that form blood cells. ETV6 contains a pointed (PNT) domain and an ETS DNA-binding domain which allow it to regulate gene expression by binding to specific DNA sequences. It functions in the nucleus where it influences cell cycle progression and differentiation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nETV6 impacts processes key to hematopoiesis and is often part of transcriptional regulatory complexes. It guides critical decisions in hematopoietic stem cells promoting lineage commitment and modulating cell cycle regulation. This protein acts as a repressor or activator depending on the context influencing target gene expression and sustaining the balance necessary for blood cell differentiation and maintenance. Its ability to interact with co-factors like HDACs (histone deacetylases) broadens its regulatory potential.\u003cbr\u003e\nPathways\u003cbr\u003e\nETV6 integrates into signaling pathways that manage cellular development and proliferation. ETV6 participates in the Ras-MAPK pathway which influences cell growth by regulating transcription of genes essential for these processes. It also interacts closely with proteins like ABL1 in this pathway modulating both kinase activity and gene control highlighting the critical connections it maintains in cell signaling networks.\u003cbr\u003e\nETV6 exhibits pivotal roles in oncogenic processes such as those observed in leukemia. Its involvement in translocation events like the ETV6-RUNX1 fusion contributes to the pathogenesis of acute lymphoblastic leukemia (ALL) by altering normal gene regulation. Additionally mutations within ETV6 link it to myelodysplastic syndromes with associated proteins in these disorders including FLT3. This connection highlights the pathogenic impact ETV6 permutations can have within hematological malignancies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855790592169,"sku":"ab258417","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258417","provider":"Iright","version":"1.0","type":"link"}