{"product_id":"abcam-ab258445","title":"Abcam, ab258445, Human HAGH (GLO2) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nHAGH KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 16 bp deletion in exon3 and 4 bp deletion in exon3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 16 bp deletion in exon3 and 4 bp deletion in exon3.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-HAGH, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGlyoxalase 2 also known as GLO2 is an important enzyme in the glyoxalase system. The protein's molecular mass is approximately 29 kDa. It catalyzes the conversion of S-D-lactoylglutathione to glutathione and D-lactic acid allowing the detoxification of methylglyoxal a byproduct of glycolysis. GLO2 is expressed in many tissues with higher levels in liver and kidney reflecting its importance in detoxifying processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGlyoxalase 2 has a significant role in cellular detoxification and maintenance of redox balance. As part of the glyoxalase system it closely works with another enzyme glyoxalase 1 to detoxify methylglyoxal. Through this interaction GLO2 helps prevent harmful effects caused by excessive levels of advanced glycation end-products (AGEs) protecting cells from potential damage.\u003cbr\u003e\nPathways\u003cbr\u003e\nGlyoxalase 2 is involved in the methylglyoxal degradation pathway within the cellular glucose metabolism process. Specifically it partners with glyoxalase 1 in this detoxification route. Additionally GLO2 contributes to glutathione metabolism where it plays a critical part alongside glutathione peroxidases which further regulate oxidative stress.\u003cbr\u003e\nGLO2 is associated with diabetes and neurodegenerative diseases. Altered levels of glyoxalase 2 impact methylglyoxal levels and have implications in diabetic complications through protein glycation. Furthermore studies indicate its potential link with Alzheimer's disease where oxidative stress and AGEs accumulation play central roles. GLO2's connection with glyoxalase 1 remains significant as both participate in these pathological states.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845186015401,"sku":"ab258445","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258445","provider":"Iright","version":"1.0","type":"link"}