{"product_id":"abcam-ab258495","title":"Abcam, ab258495, Human LDB1 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nLDB1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon3.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-LDB1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nLDB1 also known as LIM Domain Binding 1 serves an important role in transcriptional regulation. This protein with a mass of approximately 42 kDa frequently expresses in tissues such as the brain heart and spleen. LDB1 participates in forming multiprotein complexes through its interaction with LIM homeodomain proteins engaging with various co-regulators to influence gene expression.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nLIM Domain Binding 1 plays an important role in developmental processes and cell differentiation. It combines with other proteins to form a LIM complex that acts as a DNA-binding transcriptional regulator. The complex binds to enhancer elements and facilitates the activation or repression of genes critical for the development of the nervous system and cardiovascular system.\u003cbr\u003e\nPathways\u003cbr\u003e\nLIM Domain Binding 1 integrates into the Wnt signaling pathway and the TGF-beta pathway. It interacts with proteins such as TCF\/LEF and Smad serving as a cofactor that modulates transcriptional responses and influences cellular outcomes. By participating in these pathways LDB1 helps regulate cell fate decisions ensuring appropriate cellular differentiation and proliferation responses.\u003cbr\u003e\nLIM Domain Binding 1 holds relevance to leukemia and Wilms' tumor. Its dysregulation connects with disproportionate expression of key developmental genes contributing to pathogenesis. LDB1's interaction with transcription factors like SCL\/TAL1 highlights its contribution to the onset of these diseases by modulating gene expression patterns associated with these cancers.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845219471529,"sku":"ab258495","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258495","provider":"Iright","version":"1.0","type":"link"}