{"product_id":"abcam-ab258567","title":"Abcam, ab258567, Human PARP9 knockout A549 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nPARP9 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon4 and 89 bp deletion in exon4.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon4 and 89 bp deletion in exon4.,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PARP9, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPARP9 also known as ADP-ribosyltransferase diphtheria toxin-like 9 is a protein involved in cellular DNA repair and immune response. The protein has a molecular mass of approximately 88 kDa. It is notably expressed in various tissues including lymphoid tissues and immune cells suggesting a role in the immune system. PARP9 possesses domains that may facilitate interactions with other proteins playing a part in regulating cellular repair processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPARP9 enhances DNA repair mechanisms and modulates the immune response. It may work as part of larger protein complexes coordinating with other proteins to maintain genomic stability and regulate transcription. Its involvement in these complexes indicates a supportive role in cellular defense ensuring the integrity of genetic information and proper immune function. Moreover PARP9 may influence signal transduction pathways related to inflammation and cell survival.\u003cbr\u003e\nPathways\u003cbr\u003e\nPARP9 participates in critical networks that govern DNA damage response and immune signaling. It aligns with the NF-kB pathway which controls transcription of DNA cytokine production and cell survival. Additionally PARP9 associates with the innate immune response pathway influencing processes driven by related proteins like STAT1 enhancing the cellular response to external stressors. These interactions reflect its contribution to cellular adaptation and resilience.\u003cbr\u003e\nPARP9 links to conditions with underlying inflammatory or immune-related components. Its dysregulation associates with autoimmune diseases where it may influence abnormal immune activity. Additionally PARP9 has connections to certain cancers potentially through interactions with proteins like STAT3 which influences cell proliferation and survival. Understanding PARP9's role in these conditions could guide therapeutic strategies targeting abnormal protein interactions and signaling.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845178839209,"sku":"ab258567","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258567","provider":"Iright","version":"1.0","type":"link"}