{"product_id":"abcam-ab258600","title":"Abcam, ab258600, Human PPP1R16A knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nPPP1R16A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon 1 and 1 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon 1 and 1 bp deletion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PPP1R16A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe PPP1R16A protein also known as myosin light chain phosphatase target subunit 1 is part of the protein phosphatase 1 regulatory subunits. It weighs approximately 84 kDa and is mainly found in tissues like brain and skeletal muscle. It functions as a regulatory subunit modulating the activity of protein phosphatase 1 (PP1) a critical enzyme in cellular signaling.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein plays a significant role in the regulation of myosin phosphatase activity influencing muscle function. As part of a multi-subunit complex PPP1R16A interacts with PP1 to dephosphorylate myosin light chains which is essential for muscle relaxation. This process ensures proper muscle contraction and relaxation cycles in response to cellular signals.\u003cbr\u003e\nPathways\u003cbr\u003e\nProtein phosphatase activity plays key roles in both the smooth muscle contraction and Rho GTPase signaling pathways. PPP1R16A by regulating myosin light chain phosphorylation status interacts with proteins like Rho-associated coiled-coil containing protein kinase (ROCK) within these pathways. This involvement positions PPP1R16A as a vital player in pathways that govern cell motility and muscle contraction.\u003cbr\u003e\nThe dysregulation of PPP1R16A activity is associated with conditions such as hypertension and asthma. Its interaction with ROCK and misregulation in myosin phosphatase activity can contribute to abnormal muscle contractility playing a role in the pathophysiology of these diseases. Understanding this interaction helps in exploring therapeutic targets for related cardiovascular and respiratory disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845175300265,"sku":"ab258600","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258600","provider":"Iright","version":"1.0","type":"link"}