{"product_id":"abcam-ab258631","title":"Abcam, ab258631, Human RBPMS knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nRBPMS KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon2 and 44 bp deletion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon2 and 44 bp deletion in exon2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RBPMS, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRBPMS also known as RNA Binding Protein with Multiple Splicing is an important component in RNA processing. It weighs approximately 24 kDa and is widely expressed in human tissues with significant presence in the central nervous system and heart. The protein engages directly with RNA molecules impacting their stability and translation. Its structure allows it to bind specifically to RNA influencing the fate of transcripts by modulating their processing and transport.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRBPMS plays a role in the regulation of gene expression by interacting with other proteins to form ribonucleoprotein complexes. These complexes are important for the post-transcriptional management of gene expression impacting mRNA splicing and localization. RBPMS also exhibits involvement in the development of neuronal cells where it contributes to neuronal differentiation and growth by aiding in the precise processing of specific mRNA transcripts that are essential for neural functions.\u003cbr\u003e\nPathways\u003cbr\u003e\nRBPMS participates in the regulation of the mRNA splicing and transport pathways. It interacts with the spliceosome complex influencing alternative splicing events that generate diverse protein isoforms. RBPMS is associated with proteins such as SMN1 which also plays a part in mRNA processing and is integral to the spliceosomal machinery. These interactions make RBPMS a pivotal mediator in translating genetic information into functional proteins therefore affecting cellular growth and differentiation.\u003cbr\u003e\nMutations or altered expression of RBPMS have links to neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and cardiovascular disorders. In ALS RBPMS along with proteins like TDP-43 becomes misregulated contributing to disease pathogenesis by disrupting normal RNA processing and splicing patterns. In the heart compromised expression levels of RBPMS can lead to aberrant cardiac function where it might associate with proteins such as Troponin T implicating it in heart muscle contraction regulation essential for maintaining cardiac health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845070999721,"sku":"ab258631","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258631","provider":"Iright","version":"1.0","type":"link"}