{"product_id":"abcam-ab258657","title":"Abcam, ab258657, Human SEC61B knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSEC61B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SEC61B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSEC61B also known as Sec61 beta subunit or Protein Transport Protein Sec61 subunit beta is a component of the Sec61 complex. It has a mass of approximately 9 kDa. SEC61B is expressed broadly in various tissues but it is found most abundantly in the endoplasmic reticulum (ER) membrane. This protein functions mechanically as a part of the protein translocation machinery that guides nascent polypeptides into or across the ER membrane.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSEC61B assists in the regulation and positioning of the Sec61 translocon complex which includes alpha and gamma subunits. This protein is not a gatekeeper of the channel but rather stabilizes the complex and influences co-translational and post-translational translocation of proteins. It plays roles in maintaining ER homeostasis and facilitating the integration of newly synthesized proteins into the ER membrane affecting various cellular processes such as secretion and membrane biogenesis.\u003cbr\u003e\nPathways\u003cbr\u003e\nSEC61B operates in the protein translocation pathways within the ER membrane. It is integral to the secretory pathway that moves proteins from the cytoplasm into the ER lumen or membrane. Additionally SEC61B interacts with proteins like ribosome-bound signal recognition particle (SRP) to facilitate accurate protein targeting and translocation. It associates with other ER resident proteins to manage polypeptide folding and processing.\u003cbr\u003e\nSEC61B has connections to conditions that involve protein misfolding and improperly handled ER stress such as cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis misfolded proteins can trigger ER stress and cause adaptive responses. The involvement of proteins like the SEC61 complex in ameliorating ER stress highlights potential therapeutic targets. Therefore understanding SEC61B function and regulation may provide insights into therapeutic intervention for ER-stress-related disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845214982313,"sku":"ab258657","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258657","provider":"Iright","version":"1.0","type":"link"}