{"product_id":"abcam-ab258669","title":"Abcam, ab258669, Human SLC17A5 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSLC17A5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 145 bp deletion in exon1 and 49 bp deletion in exon1 and Insertion of the selection cassette in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 145 bp deletion in exon1 and 49 bp deletion in exon1 and Insertion of the selection cassette in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SLC17A5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSLC17A5 also known as sialin is a protein encoded by the SLC17A5 gene. It is a lysosomal membrane protein with an approximate molecular mass of 55 kDa. This protein facilitates the transport of sialic acid from the lysosome to the cytoplasm following degradation of sialoglycoconjugates. It gets expressed in various tissues with highest expression levels noted in the brain and kidneys.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe protein moves sialic acid a type of acidic sugar out of lysosomes important for cellular homeostasis. SLC17A5 transports not only free sialic acid but also ascorbate and other organic anions contributing to lysosomal function regulation. Though SLC17A5 functions mainly in lysosomes it can associate with membrane complexes that regulate ion transport across membranes.\u003cbr\u003e\nPathways\u003cbr\u003e\nSLC17A5 is significant within lysosomal catabolism and membrane transport pathways. In these pathways it interacts with key proteins like neuraminidase which is involved in breaking down sialoglycoconjugates. The function of sialin in moving sialic acid affects these metabolic pathways and has an impact on maintaining cellular balance.\u003cbr\u003e\nSLC17A5 has ties to free sialic acid storage disorders notably Salla disease and infantile sialic acid storage disorder (ISSD). These are neurodegenerative disorders linked to sialic acid accumulation caused by SLC17A5 malfunction. Mutations in this gene affect its transport function leading to these diseases. Additionally SLC17A5 relates to metabolic disorders by interacting with other transmembrane proteins potentially influencing disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845167861929,"sku":"ab258669","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258669","provider":"Iright","version":"1.0","type":"link"}