{"product_id":"abcam-ab258671","title":"Abcam, ab258671, Human SLC20A2 (PIT2) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSLC20A2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon2 and Insertion of the selection cassette in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon2 and Insertion of the selection cassette in exon2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SLC20A2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSLC20A2 also known as PIT2 is a sodium-dependent phosphate transporter protein with a molecular mass of approximately 80 kDa. It facilitates the uptake of inorganic phosphate into cells which is critical for various cellular functions. This protein is expressed in many tissues including the brain kidneys and lungs. In the brain SLC20A2 plays an important role in maintaining phosphate homeostasis which is necessary for neuronal activity and development.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe function of this transporter protein is to maintain phosphate balance at the cellular level. It does not form any known complex with other proteins but works by itself to regulate phosphate uptake. SLC20A2 activity is essential for normal bone mineralization and energy metabolism. The phosphate transported by SLC20A2 is used in ATP production and biological signaling processes highlighting its importance in cellular metabolism.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe role of SLC20A2 is particularly evident in the phosphate metabolism pathway. It works alongside other family proteins like SLC20A1 to regulate phosphate levels. Alteration in these pathways can impact cellular processes such as glycolysis and oxidative phosphorylation emphasizing the need for precise phosphate balance. SLC20A2 interacts with various signaling pathways that control cellular growth and differentiation showing its significance in energy and phosphate-related pathways.\u003cbr\u003e\nAbnormal SLC20A2 function has been linked to idiopathic basal ganglia calcification a disorder characterized by calcium deposit in brain regions affecting motor control. Mutations in the SLC20A2 gene can disrupt phosphate transport leading to abnormal calcium accumulation. This disorder often involves other proteins such as XPR1 which can also be implicated in phosphate transport. Understanding the role of SLC20A2 in this context helps in developing targeted therapies for better disease management.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845188440233,"sku":"ab258671","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258671","provider":"Iright","version":"1.0","type":"link"}