{"product_id":"abcam-ab258769","title":"Abcam, ab258769, Human ZNF593 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nZNF593 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 17 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 17 bp deletion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ZNF593, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nZNF593 also known as Zinc Finger Protein 593 is a protein with a mass of approximately 30 kDa. It functions as a transcriptional regulator modulating gene expression through binding DNA. ZNF593 is expressed mainly in the nucleus of cells with a concentration in tissues such as the liver kidney and heart. Its zinc finger motif suggests a role in DNA binding and regulatory functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nZNF593 is essential in managing the transcriptional activities within cells. It associates with a multiprotein complex interacting with other proteins to influence transcription. This protein plays a role in regulating cell proliferation and differentiation impacting how cells specialize and grow. Its involvement in interaction with RNA polymerase II highlights its role in gene expression regulation making it important in managing various cellular processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nThis protein enhances our understanding of cellular signaling and communication. ZNF593 is involved in the MAPK\/ERK and JAK\/STAT signaling pathways. These pathways control essential processes like cell growth differentiation and response to external stimuli. ZNF593 interacts directly with key proteins within these pathways such as ERK and STAT which further supports its role in modulating cell fate decisions and signaling cascades.\u003cbr\u003e\nZNF593’s deregulation links to certain cancers and developmental disorders. Abnormal expression of ZNF593 correlates with progression in liver cancer where it impacts cell proliferation and survival. In addition mutations affecting ZNF593 have been associated with intellectual disabilities. Its interaction with proteins like STAT in these contexts underlines its pathological significance emphasizing the importance of proper regulation for maintaining cellular health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847385469097,"sku":"ab258769","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258769","provider":"Iright","version":"1.0","type":"link"}