{"product_id":"abcam-ab258778","title":"Abcam, ab258778, Human ADCK2 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nADCK2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ADCK2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nADCK2 also known as AarF Domain Containing Kinase 2 is a protein kinase with an approximate mass of 74 kDa. It displays expression mostly in mitochondrial compartments where it exhibits its mechanical role of transferring phosphates to substrate proteins. This kinase belongs to the ABC1 (Activity of bc1 complex) family of atypical kinases which are characterized by specific serine\/threonine phosphorylation activities in various organisms.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nADCK2 is involved in cellular energy production processes. It maintains a role in the mitochondrial function influencing synthesis and maintenance of the respiratory chain complexes. Although ADCK2 is not a part of the protein complexes directly it acts as a modifier that affects the efficiency of these complexes in energy metabolism. Researchers often focus on its impact within the mitochondrial dynamics realm which is important for cellular health and activity.\u003cbr\u003e\nPathways\u003cbr\u003e\nADCK2 holds a role in both coenzyme Q biosynthesis and oxidative phosphorylation pathways. Coenzyme Q biosynthesis pathway connects ADCK2 function with several Q biosynthesis proteins such as COQ6. In oxidative phosphorylation ADCK2 contributes indirectly by affecting the electron transport chain's efficiency where it indirectly interacts with proteins such as cytochrome c oxidase components playing roles in ATP production.\u003cbr\u003e\nAltered ADCK2 expression has relations with mitochondrial myopathies and certain metabolic syndromes. These conditions often involve dysfunctions within the energy production process linking ADCK2 with proteins like COQ10 which are important in the respiratory chain. As such ADCK2 represents a potential target in understanding and addressing these metabolic imbalances and related clinical presentations.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845215047849,"sku":"ab258778","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258778","provider":"Iright","version":"1.0","type":"link"}