{"product_id":"abcam-ab258779","title":"Abcam, ab258779, Human ADPGK knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nADPGK KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 25 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 25 bp deletion in exon1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ADPGK, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nADP-dependent glucokinase commonly known as ADPGK is an enzyme that catalyzes the phosphorylation of glucose. ADPGK has a molecular mass of approximately 53 kDa. This protein primarily expresses in various tissues including liver and heart where it participates in glycolytic processes. The enzyme functions under anaerobic conditions facilitating glucose utilization when oxygen levels are low.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nADPGK facilitates energy production in cells by converting glucose into glucose-6-phosphate. This conversion is part of cellular energy metabolism especially in conditions with limited oxygen supply. While ADPGK generally acts independently under certain conditions it may interact with glucose transporters to optimize glucose uptake and metabolism. Its role is pivotal in enabling cells to adapt to metabolic demands by promoting glycolysis even without oxygen.\u003cbr\u003e\nPathways\u003cbr\u003e\nADPGK acts within the glycolytic pathway a central metabolic pathway that provides energy and biosynthetic precursors. It works alongside enzymes like hexokinase indicating a functional relationship in glycolysis initiation. ADPGK is also linked to the pentose phosphate pathway indirectly influencing NADPH production and ribose synthesis. These pathways interplay to balance energy production and anabolic reactions highlighting ADPGK's significance in cellular metabolism.\u003cbr\u003e\nADPGK influences conditions associated with impaired energy metabolism. Notably its altered function associates with certain types of cancer where abnormal glycolysis often called the Warburg effect occurs. ADPGK has connections to glucose transporters and hexokinase in these contexts impacting cancer cell metabolism. It also shows relevance in metabolic syndromes where altered glucose processing leads to various health complications. Understanding ADPGK functions helps in targeting these pathways for therapeutic interventions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845192732841,"sku":"ab258779","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258779","provider":"Iright","version":"1.0","type":"link"}