{"product_id":"abcam-ab258784","title":"Abcam, ab258784, Human ARPP19 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nARPP19 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 1 and 29 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 1 and 29 bp deletion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ARPP19, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nARPP-19 also known as cAMP-regulated phosphoprotein 19 is a protein that plays an important role in cellular processes. The protein has a molecular weight of approximately 19 kDa. Its expression can be found in various tissues with significant levels detected in the brain. ARPP-19 undergoes phosphorylation which is essential for its activity in cellular signaling.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nARPP-19 influences cell cycle regulation and neuronal signaling. The protein does not appear to be part of a larger protein complex. It interacts with protein phosphatase 2A (PP2A) modulating its activity during mitosis. This regulation helps ensure proper cell division and function. The protein also shows involvement in neurodevelopmental pathways contributing to brain development and function.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe involvement of ARPP-19 includes regulation within the cAMP signaling pathway and the mitotic spindle assembly checkpoint. It interacts with proteins like PP2A and Greatwall kinase coordinating the phosphorylation status of key mitotic substrates. ARPP-19 affects the transition from interphase to mitosis impacting the signaling cascade that controls this phase.\u003cbr\u003e\nARPP-19 has associations with cancer and neurodegenerative diseases. Aberrant phosphorylation of ARPP-19 and related proteins like PP2A can lead to mitotic errors promoting oncogenesis. In neurodegenerative contexts altered ARPP-19 function may affect neuronal signaling and plasticity contributing to conditions such as Alzheimer's disease. Understanding these interactions helps in exploring therapeutic strategies targeting these specific pathways.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845132505257,"sku":"ab258784","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258784","provider":"Iright","version":"1.0","type":"link"}