{"product_id":"abcam-ab258789","title":"Abcam, ab258789, Human ATP5J2 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nATP5MF KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 4 bp insertion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 4 bp insertion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ATP5MF, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\n'ATP5J2' also known as ATP synthase-coupling factor 6 mitochondrial is a protein involved in the synthesis of ATP the primary energy carrier in cells. This protein integrates into the ATP synthase complex specifically the F0 part where it helps regulate proton flow. ATP5J2 is approximately 11 kDa in mass and appears predominantly in mitochondria the powerhouse of cells. It expresses widely in numerous tissues highlighting an important role in cellular energy metabolism fundamental to many physiological processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nATP5J2 functions as an accessory subunit within the ATP synthase complex which is responsible for ATP production during oxidative phosphorylation. By participating in the regulation of proton translocation through the mitochondrial membrane ATP5J2 helps maintain the proton motive force necessary for ATP generation. The protein's involvement in metabolic energy conversion underlines its role in effective cellular respiration.\u003cbr\u003e\nPathways\u003cbr\u003e\nATP5J2 aligns with the oxidative phosphorylation pathway an essential metabolic route for energy production. It associates with other subunits of the ATP synthase complex such as ATP5B and ATP5O to fulfill regulatory roles in ATP synthesis. Additionally ATP5J2 has interactions within the mitochondrial electron transport chain contributing to the efficient function of this pathway and sustaining the energy requirements of cells.\u003cbr\u003e\nDefects in ATP5J2 impact mitochondrial function linking it to mitochondrial diseases which often present with muscle weakness and neural disorders. Moreover abnormalities in ATP5J2 may associate with metabolic syndromes affecting global energy metabolism. These conditions highlight potential interactions with proteins like NDUFS1 another component of the electron transport chain where disturbances can exacerbate the impact of energy deficiencies on cellular health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847389663401,"sku":"ab258789","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258789","provider":"Iright","version":"1.0","type":"link"}