{"product_id":"abcam-ab258824","title":"Abcam, ab258824, Human COL4A4 (Collagen alpha-4(IV) chain) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nCOL4A4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon29 and 2 bp insertion in exon29.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon29 and 2 bp insertion in exon29.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-COL4A4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Collagen alpha-4(IV) chain often referred to as COL4A4 is a component of type IV collagen. This protein plays a mechanical role by forming a network in the basement membranes which are thin sheets of specialized extracellular matrix materials. It possesses a significant molecular mass of approximately 185 kDa. COL4A4 is mainly expressed in tissues such as the kidney cochlea and ocular structures where it integrates with other collagen chains to provide structural support and stability.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe Collagen alpha-4(IV) chain contributes to the integrity and function of basement membranes. It forms a heterotrimeric complex with other alpha chains specifically with COL4A3 and COL4A5 which together are essential for maintaining the filtration barrier in the glomeruli of kidneys. This complex also plays roles in hearing and maintaining visual function. The structural rigidity and filtration properties provided by these collagen chains are vital for the proper functioning of these tissues.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe Collagen alpha-4(IV) chain is significantly involved in the basement membrane organization pathway. It interacts closely with other collagens and cell surface receptors to mediate cell adhesion migration and signaling processes. Within the same pathway proteins such as laminins and integrins work similarly to ensure the stability and communication between cells and the extracellular matrix. Through these interactions the COL4A4 chain ensures proper tissue organization and cellular responses to environmental changes.\u003cbr\u003e\nCOL4A4 mutations relate to Alport syndrome and thin basement membrane nephropathy. Alport syndrome is a genetic disorder characterized by kidney disease hearing loss and ocular abnormalities resulting from defects in the collagen type IV structure. In this context the COL4A4 chain interacts with COL4A3 and COL4A5 and mutations in any of these chains disrupt the collagen network causing the disease symptoms. Understanding these associations aids in diagnosing and devising potential therapeutic strategies for these disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845128442025,"sku":"ab258824","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258824","provider":"Iright","version":"1.0","type":"link"}