{"product_id":"abcam-ab258877","title":"Abcam, ab258877, Human GBP4 knockout A549 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nGBP4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon3 and 2 bp insertion in exon3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon3 and 2 bp insertion in exon3.,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-GBP4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGBP4 also known as guanylate-binding protein 4 is a member of the guanylate-binding protein family. This family is known for its role in hydrolyzing GTP to GDP and GMP an important mechanical activity that GBP4 performs. GBP4 has a mass of approximately 68 kDa. The protein is expressed in a variety of tissues but it shows higher expression in immune-related cells. It is often upregulated in response to interferon-gamma a cytokine important for immune response modulation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGBP4 plays an important role in immune response against pathogens. By disrupting microbial membranes GBP4 aids in restricting the spread of certain intracellular bacteria. It may also participate in forming a larger complex with other GBP proteins for more efficient pathogen targeting. This action is particularly significant during infections where increased GBP4 expression provides a stronger defense mechanism as part of the innate immune system.\u003cbr\u003e\nPathways\u003cbr\u003e\nGBP4 integrates functionally into interferon-gamma and JAK-STAT signaling pathways. These pathways are key components of the immune response to infections and inflammation. GBP4's role in these pathways highlights its interaction with other immune proteins such as STAT1 and other guanylate-binding proteins. These interactions highlight GBP4's importance in signaling cascades that strategize cellular immune defenses.\u003cbr\u003e\nGBP4 exhibits links to conditions like autoimmune diseases and certain infections. In autoimmune pathologies dysregulation involving GBP4 may contribute to inappropriate immune activation potentially related to proteins such as interferon-gamma. Additionally in infections GBP4 plays a role in controlling bacterial infections within macrophages often in cooperation with other members of the guanylate-binding protein family. Understanding these connections provides insights into therapeutic interventions targeting immune pathways in disease management.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845131292841,"sku":"ab258877","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258877","provider":"Iright","version":"1.0","type":"link"}