{"product_id":"abcam-ab258903","title":"Abcam, ab258903, Human HIPK3 (FIST) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nHIPK3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-HIPK3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe FIST protein also known as Found in Squamous Cell Carcinoma Transporter possesses a mass of approximately 60 kDa. It functions mechanically as a transporter protein facilitating the movement of molecules across cellular membranes. Expression of FIST occurs in various tissues with particular abundance in squamous cell carcinoma cells where it plays a role in tumor cell survival and proliferation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nFIST contributes to cellular processes by regulating the transport of specific substrates such as ions and small molecules critical for cellular homeostasis and energy balance. This protein is often part of a larger transporter complex allowing it to interact with other proteins to modulate its transport activities efficiently. Its role becomes more significant in squamous cell carcinoma where altered transport activities could contribute to the development and progression of the cancer.\u003cbr\u003e\nPathways\u003cbr\u003e\nFIST has significant involvement in the cellular energy metabolism and signaling pathways. One key pathway is the ion transport pathway where FIST interacts with several channel proteins to maintain ionic concentration and cellular excitability. Moreover it associates with ATP-binding cassette (ABC) transporters participating in the regulation of multidrug resistance which provides cancer cells with a mechanism to survive chemotherapeutic treatments.\u003cbr\u003e\nFIST has associations with squamous cell carcinoma and an increased risk of resistance to cancer therapies. Alterations in FIST expression or function can disrupt cellular ion homeostasis contributing to tumor development and progression. The protein links to the disease through its interaction with channel proteins affecting cell proliferation. Additionally its collaboration with ABC transporters in multidrug resistance pathways impacts the effectiveness of chemotherapy making the study of FIST critical in understanding and combating squamous cell carcinoma.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847384649897,"sku":"ab258903","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258903","provider":"Iright","version":"1.0","type":"link"}