{"product_id":"abcam-ab258913","title":"Abcam, ab258913, Human IFI44 knockout A549 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nIFI44 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 14 bp deletion in exon2 and 7 bp deletion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 14 bp deletion in exon2 and 7 bp deletion in exon2.,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-IFI44, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nIFI44 also known as Interferon-Induced Protein 44 is a protein encoded by the IFI44 gene. This protein has a molecular mass of approximately 49 kDa. IFI44 expresses in various tissues with elevated levels observed in immune-related cells such as leukocytes. Mechanically IFI44 is known to play a role in the antiviral defense mechanisms of cells often upregulated in response to interferon stimuli. It interacts with cytoskeleton elements to mediate its cellular functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nIFI44 participates in cellular defense against viral infections by enhancing the immune response. This protein does not function independently but forms part of interferon-stimulated gene complex working together with other proteins to combat virus replication. By regulating this complex IFI44 influences cell signaling and modulates antiviral pathways contributing to the suppression of viral propagation within host cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nIFI44 functions prominently within the interferon signaling pathway and the innate immune response pathway. It collaborates closely with other interferon-stimulated proteins such as ISG15 and MX1 which are engaged in the virus suppression. This cooperation helps orchestrate a swift immune response to external pathogenic threats by inhibiting viral gene expression and replication processes.\u003cbr\u003e\nIFI44 plays an important role in conditions associated with viral infections such as Hepatitis C and influenza. The protein’s interaction with viral defense proteins like ISG15 is significant in these disorders. Its expression levels often correlate with the severity of the disease serving as a biomarker for the infection state. Understanding IFI44’s precise role in these diseases could lead to novel therapeutic approaches and better management of viral infections.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845188505769,"sku":"ab258913","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258913","provider":"Iright","version":"1.0","type":"link"}