{"product_id":"abcam-ab258987","title":"Abcam, ab258987, Human NCOA7 knockout A549 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nNCOA7 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon11.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon11.,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-NCOA7, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNuclear receptor coactivator 7 (NCOA7) also known as ERAP plays a role in transcriptional regulation by interacting with nuclear hormone receptors to enhance their transcriptional activity. It has a molecular mass of approximately 100 kDa. NCOA7 is expressed in various tissues with significant levels in the brain and endocrine organs. The protein influences the transcriptional activity of hormone receptors by modulating chromatin structure and accessibility.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNCOA7 influences cellular processes through interactions with nuclear receptors. It forms a complex with these receptors to modulate gene expression. This protein engages in the regulation of genes that are involved in cell development and homeostasis. NCOA7's activity can influence metabolic and signaling processes that are critical for maintaining proper cellular environment and function.\u003cbr\u003e\nPathways\u003cbr\u003e\nNCOA7 associates with important biological pathways including nuclear hormone receptor signaling and lipid metabolism. It interacts with proteins like peroxisome proliferator-activated receptors (PPARs) and estrogen receptors (ERs) in these pathways. These interactions facilitate the regulation of genes involved in lipid storage energy balance and other metabolic functions.\u003cbr\u003e\nNCOA7 has implications in conditions such as breast cancer and neurodegenerative diseases. In breast cancer its interaction with estrogen receptors can influence tumor growth and development. NCOA7 is also connected to conditions like Alzheimer's disease through its influence on brain tissues potentially interacting with proteins like amyloid-beta precursor protein which is relevant to the disease pathology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847387631785,"sku":"ab258987","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258987","provider":"Iright","version":"1.0","type":"link"}