{"product_id":"abcam-ab258992","title":"Abcam, ab258992, Human NDUFB1 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nNDUFB1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-NDUFB1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNDUFB1 also known as NADH:ubiquinone oxidoreductase subunit B1 is a component of complex I in the mitochondrial respiratory chain. It plays a role in the transfer of electrons from NADH to ubiquinone. NDUFB1 has a molecular mass of approximately 12.7 kDa. Expression of NDUFB1 is observed in high energy-demanding tissues like the heart brain and skeletal muscles.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNDUFB1 functions as part of the mitochondrial complex I which is the first enzyme of the electron transport chain. Complex I is essential for energy production in cells as it facilitates the transfer of electrons and contributes to proton gradient formation across the inner mitochondrial membrane. NDUFB1 interacts with other subunits within complex I to ensure efficient electron flow impacting the overall respiratory activity and ATP synthesis.\u003cbr\u003e\nPathways\u003cbr\u003e\nNDUFB1 is involved with the oxidative phosphorylation pathway where it collaborates with other components of complex I like NDUFS7 and NDUFV1. It also associates with the mitochondrial biogenesis pathway important for maintaining mitochondrial function and energy metabolism. Through these pathways NDUFB1 helps to sustain cellular energy balance and integrate mitochondrial signaling processes.\u003cbr\u003e\nDefects in NDUFB1 have been connected to conditions such as mitochondrial complex I deficiency and Leigh syndrome. These diseases result from impaired energy metabolism leading to severe neurological and muscular symptoms. NDUFB1 dysfunction can influence the activity of proteins like NDUFA1 and NDUFS1 amplifying mitochondrial dysfunction and contributing to the progression of these disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847388319913,"sku":"ab258992","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab258992","provider":"Iright","version":"1.0","type":"link"}