{"product_id":"abcam-ab259007","title":"Abcam, ab259007, Human NUDT2 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nNUDT2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 16 bp insertion in exon2 and 1 bp deletion in exon2 and 8 bp deletion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 16 bp insertion in exon2 and 1 bp deletion in exon2 and 8 bp deletion in exon2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-NUDT2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe protein NUDT2 also known as Nucleoside Diphosphate-Linked Moiety X-Type Motif 2 is an enzyme that hydrolyzes nucleoside diphosphates. It has a molecular mass of approximately 22 kDa. This protein is expressed in various tissues including the brain liver and heart. NUDT2's mechanical function centers on its ability to regulate cellular nucleoside diphosphate levels by converting them into their monophosphate forms which is vital for the proper recycling of nucleotide pool components.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe protein plays a role in controlling intracellular hydrolysis of nucleoside diphosphates ensuring proper nucleotide balance. Although it operates independently it functions in synergy with other proteins involved in nucleotide metabolism. This precision in nucleotide regulation is essential for maintaining nucleic acid synthesis as well as energy transfer processes within the cell. NUDT2 does not form large complexes but interacts dynamically within metabolic hubs.\u003cbr\u003e\nPathways\u003cbr\u003e\nNUDT2 participates in nucleotide metabolism and salvage pathways. It contributes predominantly to pathways that recycle nucleotides essential for cellular replication and repair processes. Within these pathways NUDT2 closely interacts with enzymes such as nucleoside diphosphate kinases which play a complementary role in maintaining nucleotide balance. These interactions ensure efficient utilization of resources required for vital cellular functions.\u003cbr\u003e\nNUDT2 has been linked to conditions such as cancer and metabolic disorders. Aberrant expression or dysfunction of NUDT2 can result in nucleotide imbalance leading to problems with cell proliferation or metabolic dysregulation. In certain cancers altered expression of NUDT2 in conjunction with disrupted functions of tumor suppressor proteins can contribute to uncontrolled cell growth. Understanding NUDT2's role in these diseases can guide therapeutic strategies aimed at restoring normal nucleotide metabolism.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845175922857,"sku":"ab259007","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab259007","provider":"Iright","version":"1.0","type":"link"}