{"product_id":"abcam-ab259015","title":"Abcam, ab259015, Human OAS3 knockout A549 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nOAS3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1 and 5 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1 and 5 bp deletion in exon1.,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-OAS3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe 2'-5'-oligoadenylate synthetase 3 (OAS3) protein also known as OAS03 OAS-treate or simply OAS003 plays an important role in the innate immune response to viral infections. OAS3 is a member of the 2'-5'-oligoadenylate synthetase family and possesses a mass of approximately 125 kDa. It is ubiquitously expressed in various tissues but shows higher expression levels in liver lung and spleen. This protein is involved in the synthesis of 2'-5'-linked oligoadenylates from ATP an activity which is important for antiviral defense mechanisms in the cell.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nOAS3 contributes to the host's defense by activating RNase L a ribonuclease that degrades viral and cellular RNA to limit viral replication. OAS3 functions as part of the larger oligoadenylate synthetase family complex which enhances its ability to mediate an efficient antiviral response. The protein requires double-stranded RNA often produced during viral infections to achieve activation. This activation links the detection of viral components directly to antiviral actions effectively curtailing the spread of the virus within the host.\u003cbr\u003e\nPathways\u003cbr\u003e\nOAS3 participates in the interferon alpha\/beta signaling pathway and the 2'-5'-oligoadenylate synthetase-RNase L pathway. These pathways are essential for mediating antiviral responses in innate immunity. In these pathways OAS3 interacts with related proteins such as RNase L and other members of the OAS family like OAS1 and OAS2 which collectively enhance the immune response against viral threats.\u003cbr\u003e\nAlterations in OAS3 activity relate to diseases such as hepatitis and systemic lupus erythematosus (SLE). Abnormal OAS3 expression or function can affect host viral defense mechanisms contributing to chronic infection conditions like hepatitis. In SLE OAS3 alongside proteins like OAS1 and RNase L becomes relevant due to its role in inappropriately activating immune responses potentially leading to tissue damage. These insights into OAS3's role highlight its significance in both protective immunity and its potential involvement in autoimmunity.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845129588905,"sku":"ab259015","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab259015","provider":"Iright","version":"1.0","type":"link"}