{"product_id":"abcam-ab259049","title":"Abcam, ab259049, Human POLR1D knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nPOLR1D KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 5 bp deletion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 5 bp deletion in exon2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-POLR1D, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPOLR1D also known as RNA polymerase I and III subunit D is a component of the RNA polymerase I and III complexes. It weighs approximately 16.5 kDa. This protein is important for the transcription of ribosomal RNA (rRNA) and small RNA molecules. It is primarily expressed in cells that have high transcriptional activity such as in tissues like the pancreas thymus and spleen.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe activity of POLR1D contributes to the formation of ribosomal RNA an essential component of the ribosome which is responsible for protein synthesis. POLR1D forms part of both RNA Polymerase I and RNA Polymerase III complexes which are responsible for transcribing rRNA and other small RNAs respectively. It interacts closely with other subunits within these complexes to facilitate transcription processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nPOLR1D plays a role in the rRNA transcription pathway and the RNA polymerase I promoter opening pathway. Its function is essential within these pathways to initiate and sustain the transcription required for ribosome production. POLR1D is related to other subunits of RNA polymerases like POLR1A and POLR1B through these pathways forming an interconnected network essential for nucleolar function and RNA maturation.\u003cbr\u003e\nMalfunction or mutations within POLR1D can lead to Treacher Collins syndrome which is a disorder affecting craniofacial development. This connection highlights the importance of properly functioning RNA polymerases for developmental processes. Additionally disturbances in POLR1D have been studied in relation to leukemias where disrupted transcription processes can contribute to uncontrolled cell proliferation and cancer. POLR1D's role in these diseases highlights its importance in maintaining normal cellular operation and development.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855790297257,"sku":"ab259049","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab259049","provider":"Iright","version":"1.0","type":"link"}