{"product_id":"abcam-ab259055","title":"Abcam, ab259055, Human PPM1A knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nPPM1A KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 4 bp deletion in exon3 and Insertion of the selection cassette in exon3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 4 bp deletion in exon3 and Insertion of the selection cassette in exon3.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PPM1A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPPM1A also known as protein phosphatase 1A or PP2C-alpha functions as a serine\/threonine phosphatase. This protein plays an important role in removing phosphate groups from serine and threonine residues on proteins. The molecular mass of PPM1A is approximately 42-44 kDa. Expression of PPM1A is widespread and found in various human tissues indicating its significant involvement in multiple cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPPM1A regulates many cellular mechanisms by dephosphorylating targets and acting as a negative regulator of several signaling pathways. It modulates stress responses cell cycle progression and immune responses. PPM1A does not typically form complexes with other proteins but interacts closely with specific signaling molecules to control their activity. This regulation influences cellular homeostasis and growth in response to external and internal signals.\u003cbr\u003e\nPathways\u003cbr\u003e\nPPM1A influences the TGF-beta signaling pathway where it interacts with and deactivates SMAD proteins by dephosphorylation. It also participates in the p38 MAPK pathway. These interactions highlight its role in controlling transduction signals initiated by growth factors and stress. By regulating these pathways PPM1A maintains a balance in cellular responses to growth and environmental stresses.\u003cbr\u003e\nPPM1A links to cancer and inflammatory diseases. Its ability to modulate phosphorylation states impacts cell proliferation and immune responses critical factors in these conditions. For instance PPM1A interaction with SMAD3 in the TGF-beta pathway can influence tumor progression. Additionally its regulation of proteins such as p38 MAPK can alter inflammatory responses affecting inflammatory disease severity.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845096263849,"sku":"ab259055","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab259055","provider":"Iright","version":"1.0","type":"link"}