{"product_id":"abcam-ab259057","title":"Abcam, ab259057, Human PPP1R2 (Protein phosphatase 1 inhibitor subunit 2) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nPPP1R2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Insertion of the selection cassette in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Insertion of the selection cassette in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PPP1R2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Protein Phosphatase 1 Inhibitor Subunit 2 often abbreviated as PPP1R2 functions as a regulatory protein that inhibits the activity of Protein Phosphatase 1 (PP1). PP1 is an important enzyme in various cellular processes including cell division and glycogen metabolism. PPP1R2 has a molecular mass of approximately 20 kDa. This inhibitory protein is ubiquitously expressed throughout different tissues indicating its role in fundamental cellular functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPPP1R2 plays a critical role in cellular regulation by modulating PP1 activity. It forms a complex with PP1 in which PPP1R2 binds directly to the catalytic subunit of PP1 inhibiting its phosphatase activity. By controlling PP1 activity PPP1R2 impacts processes like muscle contraction protein synthesis and neuronal signaling making it an active participant in maintaining cellular homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nPPP1R2 functions within important signaling and dephosphorylation pathways. It is notably involved in the glycogen metabolism pathway where it regulates PP1 activity influencing glycogen synthesis and breakdown. Additionally it participates in the cell cycle regulation pathway interacting with other proteins such as PP1 to control cell proliferation and division. The regulation of these pathways by PPP1R2 highlights its importance in key cellular activities.\u003cbr\u003e\nPPP1R2's dysregulation can associate with several conditions. For instance alterations in PPP1R2 levels have implications in neurodegenerative diseases like Alzheimer's Disease since it affects neuronal protein dephosphorylation. Furthermore abnormal PPP1R2 expression can link to metabolic disorders including Type 2 Diabetes due to its role in glycogen metabolism. In these diseases its interaction with other proteins like PP1 and insulin signaling components is significant highlighting its potential as a therapeutic target.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845214949545,"sku":"ab259057","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab259057","provider":"Iright","version":"1.0","type":"link"}