{"product_id":"abcam-ab259083","title":"Abcam, ab259083, Human REXO2 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nREXO2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-REXO2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nREXO2 also referred to as RNase R or Oligoribonuclease is an enzyme with a molecular mass of around 26 kDa. It plays a significant role in the final steps of RNA decay. Mechanically REXO2 functions as an oligoribonuclease catalyzing the degradation of small ribonucleic acids into mononucleotides. This protein is primarily expressed in the mitochondria of the cell where it ensures the proper completion of RNA turnover.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe enzyme facilitates the maintenance of cellular RNA quality. REXO2 is not a part of any larger complex but its exonuclease activity allows cells to eliminate short RNA fragments which could otherwise accumulate and lead to cellular dysfunction. Efficient removal of these fragments is essential for normal mitochondrial function and energy production within the cell.\u003cbr\u003e\nPathways\u003cbr\u003e\nThis enzyme is key to ensuring RNA metabolic balance. It plays a role in the RNA degradation pathway helping to process and clear unnecessary oligoribonucleotides. While REXO2 does not directly interact with other proteins in a complex it works alongside proteins such as PNPase and RNAse II which facilitate different stages of RNA degradation and processing within the cell.\u003cbr\u003e\nREXO2 has implications in mitochondrial pathologies. Deficiencies or mutations in this enzyme can contribute to mitochondrial disorders which involve proteins such as POLG and Twinkle. Additionally its efficient RNA clearing function ties it to studies on neurodegenerative diseases where aberrant RNA processing can occur. Understanding these connections allows for deeper insight into how deviations in RNA management can translate into disease states.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845191684265,"sku":"ab259083","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab259083","provider":"Iright","version":"1.0","type":"link"}