{"product_id":"abcam-ab259085","title":"Abcam, ab259085, Human RIN1 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nRIN1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2 and Insertion of the selection cassette in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2 and Insertion of the selection cassette in exon2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RIN1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRIN1 also known as Ras and Rab interactor 1 is a protein that plays a role in intracellular signaling. It has a molecular mass of approximately 80 kDa. RIN1 interacts with both Ras and Rab GTPases and acts as a guanine nucleotide exchange factor (GEF) for these proteins. In terms of expression researchers have detected RIN1 in various tissues including the brain and embryonic fibroblasts indicating its involvement in multiple cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRIN1 modulates signaling pathways by influencing the activation state of Ras-related GTPases. It forms part of a larger signaling complex that regulates endocytosis and trafficking of receptors. This activity is essential for receptor-mediated signaling and cellular communication. By affecting these processes RIN1 contributes significantly to the regulation of cytoskeletal dynamics which impacts cell movement and morphology.\u003cbr\u003e\nPathways\u003cbr\u003e\nRIN1 is directly involved in the Ras signaling pathway which is important for cell growth and differentiation. Through its interaction with Ras proteins RIN1 influences downstream effectors like Raf kinases and PI3K which further modulate signaling cascades. RIN1 also participates in the regulation of other pathways linked to receptor internalization partnering with proteins such as Rab5 to coordinate endocytic trafficking.\u003cbr\u003e\nDisruptions or alterations in RIN1 function have been associated with certain cancers notably lung and breast cancer. These associations highlight its role in abnormal cell proliferation and survival. Moreover RIN1's interactions with Ras proteins correlate with mutations that lead to oncogenic transformations. Understanding RIN1's normal and pathological roles helps reveal potential therapeutic targets for diseases related to Ras-driven signaling dysregulation.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845179756713,"sku":"ab259085","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab259085","provider":"Iright","version":"1.0","type":"link"}