{"product_id":"abcam-ab259099","title":"Abcam, ab259099, Human SCYL1 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSCYL1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SCYL1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSCYL1 also known as Scy1 Like Pseudokinase 1 is a pseudokinase with a molecular mass of approximately 95 kDa. It localizes primarily to the cytoplasm and is strongly expressed in the central nervous system liver and kidney. The protein possesses several phosphorylation sites and a characteristic N-terminal kinase-like domain although it lacks the typical catalytic activity of kinases. SCYL1 appears to be involved in cellular processes related to protein trafficking and stability.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSCYL1 contributes to the sorting and transport of proteins between the Golgi apparatus and the endoplasmic reticulum. It interacts with coatomer complexes to facilitate vesicular trafficking ensuring proper cellular function and protein homeostasis. As part of the cellular transport machinery SCYL1 helps maintain the structural integrity of the Golgi complex and likely plays role in neurodevelopment due to its expression patterns and involvement in protein handling.\u003cbr\u003e\nPathways\u003cbr\u003e\nSCYL1 influences the Golgi-to-ER transport pathways and is involved in vesicle formation and movement. It works alongside proteins like coatomer protein complex subunits to maintain efficient vesicular transport within the cell. Through these pathways SCYL1 connects to broader cellular functions like protein sorting and endosome-to-Golgi retrieval contributing to the systematic recycling and allocation of proteins across the cellular framework.\u003cbr\u003e\nSCYL1 mutations link the protein to spinocerebellar ataxia type 21 and the hepatic disorder 3C syndrome. These conditions highlight the protein's connections to neurological and liver functions reflecting its significant role in these organs. Additionally interactions with proteins like TDP-43 suggest potential contributions to neurodegenerative processes when misregulated. Understanding SCYL1's role in these diseases provides insight into its importance for normal cellular functioning and the consequences of its dysfunction.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855780663465,"sku":"ab259099","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab259099","provider":"Iright","version":"1.0","type":"link"}