{"product_id":"abcam-ab259101","title":"Abcam, ab259101, Human SEC13 (SEC13L1) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSEC13 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 2 bp deletion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 2 bp deletion in exon2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SEC13, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSEC13L1 also known as SEC13 or SEC13-like is a protein encoded by the SEC13L1 gene. It is a component of a complex involved in vesicle trafficking. The protein has an approximate molecular mass of 37 kDa and is known to be expressed in various tissues across the human body indicating a broad functional role. SEC13L1 is an integral part of the coated vesicles that mediate protein transportation between the endoplasmic reticulum and the Golgi apparatus.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAs a part of the SEC13 complex SEC13L1 serves an essential function in the process of vesicular transport. This protein forms a core element of the COPII coat complex which is a multi-protein assembly responsible for the budding of transport vesicles from the endoplasmic reticulum. Besides its role in protein transport SEC13L1 also participates in maintaining the architecture of the nuclear pore complex which is critical for nucleocytoplasmic transport.\u003cbr\u003e\nPathways\u003cbr\u003e\nSEC13L1 plays an important role in the secretory pathway and is involved in the ER-Golgi transport pathway. It associates closely with proteins like SEC24 and SEC23 which work together in the COPII coat formation. The interaction with these proteins is important for the selection of cargo proteins that are exported in COPII vesicles. Furthermore SEC13L1 is also involved in the regulation of nucleocytoplasmic transport pathways alongside proteins such as NUP107 as part of the nuclear pore complex degradation.\u003cbr\u003e\nSEC13L1 has been associated with nephrotic syndrome where its dysregulation may impact protein trafficking and cellular homeostasis. It also plays a role in cancer where the protein's involvement in nuclear pore functions and vesicle formation may affect cell proliferation and survival. SEC13L1 links to disorders involving nucleocytoplasmic transport often interacting with nucleoporins such as NUP133 that may be implicated in pathogenesis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845192994985,"sku":"ab259101","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab259101","provider":"Iright","version":"1.0","type":"link"}