{"product_id":"abcam-ab259152","title":"Abcam, ab259152, Human SPCS1 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSPCS1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 1 and 2 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 1 and 2 bp deletion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SPCS1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSignal peptidase complex subunit 1 (SPCS1) also known as SPC18 is a protein with an approximate mass of 22 kDa. It forms a part of the signal peptidase complex in the endoplasmic reticulum of eukaryotic cells. This protein assists in the cleavage of signal peptides from nascent proteins being synthesized. SPCS1 is ubiquitously expressed across various tissues which indicates its significance in general cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSPCS1 supports the proper processing and maturation of secretory and membrane proteins. As a constituent of the signal peptidase complex SPCS1 participates directly in the co-translational removal of signal peptides. This functionality is integral for protein targeting and trafficking ensuring that proteins reach their intended locations within the cell or are secreted outside.\u003cbr\u003e\nPathways\u003cbr\u003e\nSPCS1 is involved in the protein processing pathway within the endoplasmic reticulum. This pathway plays a role in maintaining cellular protein homeostasis. The function of SPCS1 makes it relevant to the unfolded protein response pathway indirectly influencing pathways that involve stress response and protein folding. Proteins like SEC61 which form the translocon complex work closely with SPCS1 in these pathways to facilitate protein synthesis and translocation across the ER membrane.\u003cbr\u003e\nDisruptions in the function of SPCS1 can relate to certain neurodegenerative diseases due to its role in protein processing. Improper protein cleavage and folding could contribute to conditions such as Alzheimer's disease. Additionally the dysregulation of SPCS1 is connected to cancer where anomalies in protein maturation pathways may lead to uncontrolled cell growth. In these contexts SPCS1 abnormalities may be associated with other proteins such as tau in Alzheimer's disease and various oncogenes or tumor suppressor proteins in cancer.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845184868521,"sku":"ab259152","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab259152","provider":"Iright","version":"1.0","type":"link"}