{"product_id":"abcam-ab259153","title":"Abcam, ab259153, Human SPCS3 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSPCS3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 344 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 344 bp deletion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SPCS3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSPCS3 also known as Signal Peptidase Complex Subunit 3 functions mechanically as a component of the signal peptidase complex which is responsible for cleaving the signal peptides from nascent proteins. It plays an important role in the protein maturation process within the endoplasmic reticulum. The protein is approximately 15 kDa in mass and is highly expressed in tissues such as the liver and pancreas. Its expression is critical in cells where active protein synthesis occurs.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe signal peptidase complex including SPCS3 is involved in the processing and maturation of secretory and membrane proteins. SPCS3 forms part of a larger multisubunit complex that ensures proteins assume their proper functional forms. By cleaving signal peptides SPCS3 facilitates the proper localization and function of proteins within the cell. In doing so it contributes to cellular processes that depend on effective signal peptide processing such as growth and development.\u003cbr\u003e\nPathways\u003cbr\u003e\nSPCS3 plays a significant role in the protein processing pathway in the endoplasmic reticulum. It interacts with other proteins involved in this pathway such as SPCS1 and SEC61 ensuring the correct translocation and maturation of newly synthesized polypeptides. The efficient functioning of SPCS3 in these pathways is important for maintaining cellular homeostasis and supporting protein synthesis and secretion.\u003cbr\u003e\nResearch links SPCS3 to conditions involving protein misfolding and secretion dysfunction such as cystic fibrosis and some forms of diabetes. In cystic fibrosis the malfunction of SPCS3 can lead to improper processing of the CFTR protein exacerbating disease symptoms. The protein's role in these diseases connects it to other factors involved in the pathology such as the CFTR protein. Understanding how SPCS3 influences these disorders can help develop targeted therapeutic strategies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847383961769,"sku":"ab259153","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab259153","provider":"Iright","version":"1.0","type":"link"}