{"product_id":"abcam-ab259159","title":"Abcam, ab259159, Human STK16 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSTK16 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon3.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-STK16, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSTK16 also known as Serine\/threonine-protein kinase 16 or KRCT is a protein kinase that has a mass of approximately 33 kDa. It phosphorylates proteins on serine and threonine residues playing a role in several cellular processes. STK16 is expressed in many body tissues with higher levels detected in the brain liver and spleen. Its widespread expression suggests it has a function in different cellular contexts.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSTK16 influences various processes at the cellular level. It participates in vesicular trafficking and Golgi function ensuring proper sorting and delivery of proteins within cells. Although not typically seen as part of large complexes it interacts with several other proteins to exert its biological roles. The protein’s activity affects cell cycle progression and can influence cellular growth and motility.\u003cbr\u003e\nPathways\u003cbr\u003e\nSTK16 integrates into key regulatory networks such as the Golgi-associated vesicle budding pathway and cell signaling pathways. These pathways are important for maintaining cellular organization and communication. STK16’s interactions with proteins like COPII in vesicle trafficking and possibly components of the MAPK signaling cascade highlight its connectivity within cellular pathways and its regulatory potential.\u003cbr\u003e\nSTK16 has been associated with conditions related to cell cycle dysregulation and tumor growth. Dysfunctions in STK16 expression or activity might contribute to the progression of cancers such as breast cancer by impacting cell proliferation and survival. Other kinases like Akt may interact with STK16 or similar pathways influencing disease pathways and offering potential therapeutic intervention points.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855779221673,"sku":"ab259159","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab259159","provider":"Iright","version":"1.0","type":"link"}