{"product_id":"abcam-ab260051","title":"Abcam, ab260051, Human THBS2 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman THBS2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human THBS2 with a sensitivity of 0.024 ng\/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture media, Heparin Plasma, Citrate plasma, Milk, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human, Rhesus monkey,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.024 ng\/mL,\u003cbr\u003e\nRange:0.09 - 6 ng\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman THBS2 ELISA Kit (ab260051) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of THBS2 protein in cit plasma, edta plasma, hep plasma, milk, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human THBS2 with 0.024 ng\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n- Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n- High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n- Fully validated in biological samples\u003cbr\u003e\n- 96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003cbr\u003e\nTHBS2 (Thrombospondin-2) is a secreted cell adhesion glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. It is a ligand for CD36 which mediates antiangiogenic properties.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThrombospondin 2 also known as TSP2 is a glycoprotein with a molecular weight of approximately 150 kDa. It is an extracellular matrix protein found in a variety of tissues including vascular tissues and connective tissues. Expression occurs in fibroblasts endothelial cells and certain types of immune cells. TSP2 plays a role in cell-to-cell and cell-to-matrix interactions through its multi-domain structure allowing it to bind with a range of cellular receptors and matrix components.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTSP2 modulates angiogenesis the process of new blood vessel formation. It functions as an inhibitor of angiogenesis contributing to the regulation of tissue repair and remodeling. TSP2 does not operate alone; it is part of a larger complex within the extracellular matrix that also involves other thrombospondins like Thrombospondin 1 with which it shares some functional similarities. These thrombospondins collaborate to influence cell adhesion and migration.\u003cbr\u003e\nPathways\u003cbr\u003e\nTSP2 participates significantly in the TGF-β signaling pathway and the regulation of vascular endothelial growth factor (VEGF) activity. Through the TGF-β pathway TSP2 influences extracellular matrix remodeling and tissue fibrosis. Additionally its interaction with VEGF modulates angiogenesis where TSP2 acts as an antagonist counterbalancing the effects of VEGF. This interaction links TSP2 with regulatory mechanisms involving proteins like integrins which play roles in the cellular attachment to the matrix.\u003cbr\u003e\nTSP2 connects notably with cancer and fibrosis. In cancer its angiogenesis-inhibiting properties can affect tumor growth and metastasis by limiting blood supply to the tumor a process that involves its interaction with matrix metalloproteinases. In fibrosis especially pulmonary and hepatic forms TSP2 influences tissue scarring and repair through its oppression of angiogenesis and interaction with the TGF-β pathway affecting fibrotic protein expression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843577335977,"sku":"ab260051","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab260051","provider":"Iright","version":"1.0","type":"link"}