{"product_id":"abcam-ab261658","title":"Abcam, ab261658, Human GJA1 knockout HEK-293 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nGJA1 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR\/Cas9; X = 1 bp insertion, 11 bp deletion, 2 bp deletion.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Next Generation Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by CRISPR\/Cas9; X = 1 bp insertion, 11 bp deletion, 2 bp deletion\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-GJA1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nConnexin 43 also known as GJA1 is a protein that plays an important role in cell communication through gap junctions. It has a molecular weight of approximately 43 kDa. Connexin 43 gets expressed in various tissues including the heart and brain where it facilitates cellular signaling. This protein integrates into cell membranes to form gap junction channels allowing direct transfer of ions and small molecules between adjacent cells. Researchers often use techniques such as Western blot to detect Connexin 43 expression levels in studies of cellular communications.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein facilitates electrical and chemical coupling between cells by forming intercellular channels. These channels arise from the assembly of six Connexin 43 subunits into hemichannels which dock with hemichannels on neighboring cells. This protein has a critical component in the cardiac and neural tissues where rapid communication is essential for proper function. Connexin 43 engages in complex assemblies with other connexins enhancing its role in synchronizing cell activities.\u003cbr\u003e\nPathways\u003cbr\u003e\nConnexin 43 significantly influences signal transduction processes. It acts within the MAPK signaling pathway which impacts cellular responses such as growth and differentiation. Additionally this protein plays a role in calcium signaling a pathway important for muscle contraction and neurotransmitter release. Interacting proteins like Connexin 30 and Connexin 45 further regulate these pathways affecting the amplitude and duration of signaling.\u003cbr\u003e\nConnexin 43 has been linked to cardiac arrhythmias and some forms of cancer. Mutations or altered expression of Connexin 43 can disrupt the electrical conduction in cardiac tissue leading to arrhythmogenic conditions. Research suggests an association with proteins like ZO-1 and Connexin 37 which may affect cell proliferation and migration in cancerous tissues. Understanding these connections highlights Connexin 43's potential as a target for therapeutic interventions in related diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845126508713,"sku":"ab261658","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab261658","provider":"Iright","version":"1.0","type":"link"}