{"product_id":"abcam-ab261776","title":"Abcam, ab261776, Human PMS2 knockout HeLa cell line","description":"\u003cp\u003eSize: 1000000Cells \/ vial \/ 2 x 1000000Cells \/ vial\u003cbr\u003e\nPMS2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 16 bp deletion in exon 2 and 2 bp deletion in exon 2 and Insertion of the selection cassette in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 16 bp deletion in exon 2 and 2 bp deletion in exon 2 and Insertion of the selection cassette in exon 2,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PMS2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPMS2 also known as Postmeiotic Segregation Increased 2 or PMS2 MutL homolog is a protein that participates in DNA mismatch repair. It weighs approximately 96 kDa and often identifies as a member of the MutL protein family. PMS2 is ubiquitously expressed in the body with higher abundances in tissues that undergo rapid proliferation or possess a high mitotic index.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPMS2 operates as part of the DNA mismatch repair (MMR) complex. It collaborates with other MutL homologs including MLH1 to form a heterodimer which is essential for repairing DNA replication errors. It safeguards genomic integrity and prevents mutations from accumulating in dividing cells serving important functions in cellular viability and genetic stability.\u003cbr\u003e\nPathways\u003cbr\u003e\nPMS2 is involved in the DNA damage response and cell cycle regulation. The protein plays a vital role in the mismatch repair (MMR) pathway. PMS2 partners primarily with MLH1 within this pathway and both proteins work in conjunction to recognize and initiate repair on erroneous DNA sequences that emerge during replication preventing illegitimate recombination and chromosomal rearrangements.\u003cbr\u003e\nPMS2 mutations occur frequently in Lynch syndrome an inherited cancer predisposition disorder and Turcot syndrome a condition associated with colorectal cancer and brain tumors. MLH1 frequently associates with PMS2 in these disorders as defects in either protein can impair mismatch repair leading to an increased risk of cancer.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845100621993,"sku":"ab261776","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab261776","provider":"Iright","version":"1.0","type":"link"}