{"product_id":"abcam-ab263106","title":"Abcam, ab263106, Human ATRAID (C2orf28) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nATRAID KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 11 bp deletion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 11 bp deletion in exon2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ATRAID, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nC2orf28 also called FLJ20492 is a protein encoded by a gene on chromosome 2 open reading frame 28. This protein has a molecular mass of approximately 20 kDa. It is chiefly expressed in various human tissues including the brain heart and liver. Mechanically C2orf28 interacts with other cellular components although its precise role in cellular processes is not fully defined yet.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe C2orf28 protein contributes to intracellular signaling and regulatory processes. Although this protein is not widely recognized as part of a complex it participates in maintaining cellular homeostasis which involves a variety of regulatory functions. Due to its expression pattern and regulatory roles it is considered to have an impact at the cellular level.\u003cbr\u003e\nPathways\u003cbr\u003e\nC2orf28 engages in several cell signaling and metabolic pathways. It notably influences pathways related to cellular stress responses and metabolic regulation. The protein may interact with other mediators like AKT1 and MTOR which are essential in these pathways. C2orf28's involvement in such pathways underlines its role in modulating cellular dynamics.\u003cbr\u003e\nC2orf28 is linked with neurodegenerative conditions and metabolic disorders. Research indicates its potential involvement in Alzheimer's disease due to its expression in the brain and interaction with proteins like tau. Additionally its role in metabolic regulation suggests a connection to disorders such as diabetes where it may interact with proteins involved in insulin signaling. Further studies could elucidate the precise mechanisms of these associations.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845191356585,"sku":"ab263106","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263106","provider":"Iright","version":"1.0","type":"link"}