{"product_id":"abcam-ab263164","title":"Abcam, ab263164, Human DCTPP1 (XTP3TPA) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nDCTPP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-DCTPP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nXTP3TPA also known as Diecloropyrophosphatase is part of a larger family of proteins involved in pyrophosphate hydrolysis. This protein has a mass of approximately 65 kDa. XTP3TPA expression occurs in several tissues with higher levels in liver and kidney. These tissues play a major role in metabolic processes which would likely relate to this protein's functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nXTP3TPA functions to regulate cellular energy. These proteins also influence metabolic efficiency by hydrolyzing pyrophosphate to inorganic phosphate. XTP3TPA often operates within larger protein complexes to enhance its enzymatic role. This activity represents a common aspect of cellular metabolism impacting processes like ATP production.\u003cbr\u003e\nPathways\u003cbr\u003e\nXTP3TPA integrates into the ATP synthesis and nucleotide metabolism pathways. Within these pathways XTP3TPA sometimes collaborates with proteins such as ATP synthase coordinating energy production and utilization. Its presence in these pathways ensures proper energy allocation which is important for cellular survival and function.\u003cbr\u003e\nXTP3TPA abnormalities link to metabolic disorders such as type 2 diabetes and metabolic syndrome. Disrupted function or expression of XTP3TPA might impact proteins like AMP-activated protein kinase (AMPK). This disruption can lead to metabolic imbalances necessitating more research into XTP3TPA for potential therapeutic targets.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855779287209,"sku":"ab263164","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263164","provider":"Iright","version":"1.0","type":"link"}